Шинжлэх ухаан Нөхөн төлжүүлэх эмчилгээний шинэ үеийн эхлэл -iPS эс хэрэглээнд-
  • 2017/05/01
  • 306
  • 73

Японы хамгийн том судалгааны төв болох Рикэн-ий “Нүдний торлог бүрхүүлийн нөхөн төлжүүлэх эмчилгээний судалгааны төсөл”-ийн багийнхан энэ 3 сарын 28-нд анхны өвчтөндөө эс шилжүүлэх мэс засал хийжээ. Анхны өвчтөн нь 60 настай эрэгтэй бөгөөд 6 хүний бүрэлдэхүүнтэйгээр мэс засал хийж, төлөвлөсөн ёсоор нэг цагийн дотор мэс заслыг дуусгажээ. Уг мэс засалд Киото их сургуулиас хүлээн авсан iPS эсээс гаргаж авсан, нүдний торлог бүрхүүлийн эпидермийн 650 мянга орчим эсийг агуулах шингэнийг шилжүүлжээ. Энэхүү мэс засал нь iPS эс ашигласан хоёр дахь нөхөн төлжүүлэх эмчилгээ болж байгаа юм.

Анхны нөхөн төлжүүлэх мэс засал нь хэзээ явагдав?

Анхны мэс засал нь 2014 оны 9 сарын 12-нд амжилттай болж өндөрлөсөн юм. Тус мэс засал нь хөгшрөлтөөс үүдэх шар толбоны өөрчлөлт гэгдэх нүдний өвчний эмчилгээнд зориулан хийгдсэн байна. Энэ мэс засалд өвчтөний өөрийнх нь арьсны эснээс гаргаж авсан iPS эсийг ашиглажээ. Энэ нь iPS эсийг ашигласан нөхөн төлжүүлэх эмчилгээний аюулгүй байдлыг шалгахад чухал үр дүн өгсөн мэс засал байсан юм. 2015 оны 10 сарын 2-нд тус өвчтний эмчилгээний үр дүнг тайлагнахад, мэс заслаас нэг жил өнгөрсний дараагаар хавдар зэргийн өөрчлөлт харагдалгүй, анхны мэс засал гэхэд сайн үр дүн үзүүлсэн аж. Өвчтөний зүгээс өмнөхөөсөө илүү тод, өргөн хүрээтэйгээр харж чадах болсондоо баяртай байгаагаа мөн дуулгасан юм. Энэхүү судалгаа нь дэлхий нийтэд цацагдан, тус төслийн удирдагч Такахаши Масаё нь Английн Натур шинжлэх ухааны сэтгүүлийн оны онцлох 10 эрдэмтэний нэгд шалгарсан байна.

ES эсээс iPS эс рүү

 Такахаши Масаё гуай нь 1992 онд Киото их сургуульд анагаах ухааны докторын зэргийг хамгаалж, 1995 онд тархины мэс заслын эмч нөхрийгөө даган Америк явсан байна. Тэнд очоод тэр үедээ дөнгөж олдоод байсан мэдэрлийн тулгуур эсийн талаар олж мэдэн, нүдний торлог бүрхүүлийн эмчилгээгүй өвчнийг эмчлэх боломжийг олж харсан байна. 2000 оноос ES эсэд анхаарлаа хандуулан, тус эсээс мэдрэлийн эс гаргаж авах судалгаа хийж байсан Рикэний судлаач Сасаи Ёшики гуайтай хамтарч, анх удаа ES эсээс мэдрэлийн торлог бүрхүүлийг гаргаж авчээ. Үүнээс хойш төд удалгүй 2006 онд Яманака Шиня гуай iPS эсийг нээж (2012 онд үүгээрээ Нобелийн шагнал хүртсэн), Такахаши Масаё гуай нь ES эсээс хэрэглэхэд илүү тохиромжтой iPS эс рүү шилжсэн байна.
Миний бие анхны мэс заслын дараах жил болох 2015 оны 5 сард Такахаши Масаё гуайн лекцийг сонсох завшаан тохиосон юм. Тэрбээр “Хүмүүс iPS эс олдоод ердөө наймхан жилийн дотор хүн дээрх эмчилгээ эхэлсэнд гайхдаг. Гэхдээ энэ судалгаа нь ES эсээс эхэлсэн судалгаа юм” хэмээж, хүн дээрх эмчилгээ явагдах хүртэл судалгаанаас гадна өөр олон хүчин зүйл хэрэгтэй байсныг дурдсан юм.

Энэ удаагийн мэс заслын онцлог

Энэ удаагийн мэс засал нь анхны iPS эс ашигласан мэс засал байгаагүй байсан юм бол, өмнөхөөсөө юугаараа онцлог вэ? Энэ нь тус хүний өөрийн эс бус бусдын эсийг ашигласнаараа, анхны өөрийн бус эсийг ашигласан нөхөн төлжүүлэх эмчилгээ болж байгаа юм. Тэгвэл яагаад одоог хүртэл чадаагүй вэ? Ерөнхийдөө хүний биед өөрийн бус зүйл орж ирж байгаа тул, дархлааны систем гадны зүйл гэж танин, устгах урвалыг явуулна. Ингэснээрээ зөвхөн шинээр оруулж ирсэн эс бус, түүний эргэн тойрны эсэд ч хөнөөл учруулах тул  өвчтөн бүр мөсөн сохрох ч аюулыг дагуулж болно. Гэтэл энэ удаагийн мэс засал нь энэ асуудлыг шийдсэн байна. HLA дархлааг нь тааруулсан iPS эс хэрэглэснээрээ шийдэлд хүрчээ. Энэ нь ерөнхийдөө бол А бүлгийн цустай хүнд О бүлгийн цус сэлгэж болдогтой төстэй шалтгаантай юм.

Бусдын эснээс гаралтай iPS ашиглах давуу ба сул талууд

Давуу тал нь:

  1. Цаг алдахгүй. Өөрийн эсийг ашиглана гэвэл, эхлээд тус хүний эсний дээжийг авч iPS эсийг гаргаж авах шаардлагатай. Цаашлаад түүнийгээ хэрэгтэй эс болгож, түүнийхээ аюулгүй байдлыг шалгах гээд бараг хагас жил орчим шаардагдах болно. Лабораторид бэлтгэсэн бусдын эсийг ашиглавал энэ хугацааг хэмнэх болно.
  2. Өртөг хэмнэнэ. Өвчтөн тус бүрийн iPS эсийг хийнэ гэдэг нь хүн хүчний, тоног төхөөрөмжийн зэрэг зардлууд гарч, маш өндөр өртөгтэй эмчилгээ болох юм. Энэ удаагийн эмчилгээ нь энэ өртгийг багасгах технологийг туршсан гэж хэлж болно.

Сул тал нь:

  1. Хэд хэдэн төрлийн донор болох iPS эсийг бэлдэх шаардлагатай. Үүний талаарх нарийн зүйлийг дараагийн нийтлэлдээ тайлбарлах болно.
  2. Цусны бүлэгт ховор гэж байдаг шиг HLA-д ч ховор гэж бий. Тиймээс HLA-н төрөл нь донор болгон хадгалсан эстэй бүгдэнтэй нь таарахгүй бол өөрийн эсийг хэрэглэхээс өөр аргаагүй болох болно.

Энэ удаагийн нийтлэлдээ iPS эсийн хэрэглээний сүүлийн үеийн шинэхэн мэдээнээс дуулгалаа. Дараагийн нийтлэлдээ тус эмчилгээний талаар бага зэрэг гүнзгийрүүлэн тайлбарлах болно. Миний хувьд биохими, эсийн биологийн чиглэлээр суралцдаг ч, анагаахын мэдлэг хомс тул зарим анагаахын үгсийг махчилж орчуулсан байгаа. Тиймээс буруу орчуулагдсан байвал хүлцэл өчье.
 
Бусад энэ сэдэвтэй холбоотой мэдээллийг эндээс сонирхоорой.

“Уучлаарай, генээ засуулах гэсэн юм?”

Хавдрын тулгуур эс

STAP эс гэж үнэхээр оршдог уу?

 

Ө. Нарандулам

Ашигласан материал:

  1. Рикэн-ий нүдний торлог бүрхүүлийн нөхөн төлжүүлэх эмчилгээний судалгааны төслийн багийн веб хуудас

http://www.cdb.riken.jp/research/laboratory/takahashi.html

  1. Японы википедиа хуудсын Такахаши Масаё гуайн танилцуулга

https://ja.wikipedia.org/wiki/%E9%AB%98%E6%A9%8B%E6%94%BF%E4%BB%A3#cite_note-nature2014-5-1

Сэтгэгдэлүүд ( 73)

  • air jordan 13 - 2018/11/14

    I want to get across my affection for your kind-heartedness for folks who need assistance with in this situation. Your special dedication to getting the solution up and down was extremely advantageous and have without exception permitted those much like me to get to their pursuits. Your new informative advice signifies this much a person like me and substantially more to my office colleagues. Regards; from all of us.

  • vans outlet - 2018/11/12

    I precisely wished to thank you so much once again. I am not sure the things I would've created in the absence of those tricks shown by you about this topic. It truly was an absolute frightful setting in my circumstances, but seeing your specialised mode you resolved it forced me to weep over delight. I'm happier for this assistance and thus expect you recognize what a great job your are putting in educating the rest by way of your websites. I am sure you haven't encountered all of us.

  • Adidas NMD R1 Primeknit Tri-Color Light Grey White - 2018/11/12

    I enjoy you because of each of your work on this site. Gloria enjoys making time for internet research and it is easy to understand why. Many of us hear all of the powerful method you deliver important guidance through the web site and in addition recommend contribution from others about this content so our favorite girl is without question being taught a great deal. Take advantage of the remaining portion of the new year. You are performing a wonderful job.

  • adidas nmd - 2018/11/11

    Thanks for all of your work on this web site. My daughter enjoys making time for internet research and it's really simple to grasp why. We all notice all regarding the powerful form you make invaluable techniques by means of the website and as well as cause response from other ones on this subject while our own girl is always becoming educated a lot of things. Enjoy the rest of the year. You have been doing a great job.

  • curry 4 shoes - 2018/11/11

    I needed to send you a little observation to help say thank you as before with your striking principles you have contributed in this article. It has been certainly pretty generous of you to offer freely exactly what a few individuals could have marketed for an electronic book to end up making some profit for themselves, especially since you could possibly have done it in case you considered necessary. The techniques additionally served as the easy way to be certain that most people have similar keenness similar to mine to see somewhat more pertaining to this matter. I'm sure there are numerous more pleasurable periods up front for people who browse through your website.

  • nike hyperdunk - 2018/11/10

    I precisely had to thank you so much yet again. I am not sure what I could possibly have made to happen in the absence of the type of suggestions provided by you on my subject matter. Certainly was an absolute intimidating circumstance in my view, nevertheless witnessing the specialised way you treated that made me to leap over happiness. Now i am grateful for the work and then expect you find out what an amazing job you were getting into educating people all through your website. More than likely you've never encountered any of us.

  • goyard - 2018/11/08

    I definitely wanted to write down a quick remark to thank you for all the lovely information you are placing here. My particularly long internet look up has at the end of the day been compensated with really good information to talk about with my relatives. I would mention that we website visitors actually are unequivocally endowed to exist in a useful place with many brilliant people with beneficial strategies. I feel truly blessed to have seen the weblog and look forward to plenty of more excellent moments reading here. Thanks a lot once more for everything.

  • terringKt - 2018/11/08

    Это не имеет смысла. --- Как так? интересные кафе, интересные вакансии и здесь интересные парадоксы сайт там тут тут здесь источник сайт на этом сайте сайт тут

  • converse outlet - 2018/11/07

    Thanks a lot for giving everyone an extremely brilliant opportunity to read critical reviews from here. It's usually so ideal plus packed with a great time for me and my office fellow workers to visit the blog really 3 times in a week to study the latest stuff you have. And of course, I am actually motivated with the surprising information you give. Selected 1 facts on this page are certainly the very best we have all had.

  • lebron 15 - 2018/11/06

    Thanks a lot for giving everyone a very marvellous opportunity to discover important secrets from this website. It can be so excellent plus jam-packed with fun for me personally and my office fellow workers to search your website minimum 3 times a week to see the newest stuff you have got. And lastly, we're always amazed with the unique tricks you serve. Certain 2 ideas in this posting are easily the most effective I have ever had.

  • Adidas NMD Runner Unisex Golden Brown - 2018/11/06

    I precisely wanted to thank you so much once again. I do not know what I would have done in the absence of the entire ways documented by you over such a situation. Certainly was a real frustrating setting in my view, however , taking a look at a skilled style you handled that took me to leap with happiness. I'm just happier for the guidance as well as wish you recognize what an amazing job you have been accomplishing educating the others using your websites. Most likely you haven't got to know all of us.

  • michael jordan shoes - 2018/11/05

    I have to show some thanks to you for bailing me out of this situation. Just after looking throughout the online world and finding recommendations which were not helpful, I figured my life was well over. Existing without the strategies to the difficulties you've solved by way of your blog post is a crucial case, as well as those which may have in a negative way affected my career if I had not encountered your blog. Your personal capability and kindness in touching every part was crucial. I don't know what I would've done if I had not come across such a step like this. I am able to at this moment relish my future. Thank you very much for your skilled and effective help. I won't be reluctant to refer your web sites to anyone who should get care on this area.

  • nike polo - 2018/11/04

    I needed to send you one little note to say thanks a lot once again for those marvelous pointers you have shared here. This is certainly open-handed of you to provide publicly all that many individuals would've marketed for an electronic book in order to make some cash for their own end, certainly given that you might have tried it if you ever desired. These pointers likewise worked to be the fantastic way to fully grasp the rest have the same fervor really like mine to figure out a little more on the subject of this problem. I am certain there are some more pleasurable instances ahead for many who read your blog.

  • hermes belt - 2018/11/03

    I have to express thanks to the writer for rescuing me from this particular scenario. After researching through the the web and coming across tricks that were not productive, I was thinking my life was over. Being alive without the answers to the difficulties you've sorted out through the guide is a crucial case, and ones which could have adversely damaged my career if I had not noticed your blog post. Your own mastery and kindness in touching all areas was crucial. I don't know what I would have done if I hadn't encountered such a point like this. I'm able to at this time look forward to my future. Thank you very much for this skilled and result oriented help. I won't think twice to recommend your blog post to any person who would like tips about this issue.

  • zx flux - 2018/11/02

    I am only writing to make you know what a useful experience my wife's child found reading through your webblog. She discovered several pieces, including how it is like to possess an awesome teaching nature to have most people without hassle comprehend a variety of grueling things. You undoubtedly exceeded people's expected results. Thank you for rendering such priceless, trusted, revealing not to mention easy guidance on the topic to Janet.

  • golden goose sneakers - 2018/11/02

    I simply needed to thank you so much yet again. I'm not certain what I would have handled in the absence of these advice shown by you about my field. This has been the fearsome situation in my position, however , spending time with this skilled form you managed that took me to jump for fulfillment. I will be happier for this guidance and as well , have high hopes you are aware of a powerful job you are putting in instructing most people through a blog. Most likely you haven't got to know all of us.

  • yeezy shoes - 2018/11/01

    My wife and i were really relieved Ervin managed to do his web research through the entire ideas he acquired when using the blog. It is now and again perplexing to just continually be freely giving information and facts which people have been selling. And we acknowledge we've got the blog owner to thank because of that. The specific explanations you made, the straightforward blog navigation, the friendships you assist to create - it is everything astonishing, and it's really leading our son and the family imagine that that subject is brilliant, and that's particularly pressing. Many thanks for all!

  • tresrigef - 2018/10/31

    Bravo, this remarkable idea is necessary just by the way --- In my opinion you are not right. I am assured. I suggest it to discuss. Write to me in PM, we will communicate. porn online massage, porn online game, here porn online 3gp here here here here here here here here here here

  • verbroki - 2018/10/31

    It — is improbable! --- I recommend to you to come for a site where there is a lot of information on a theme interesting you. comix porn online, online porn young, here teacher porn online here here here here here here here here here here

  • contmanfSah - 2018/10/31

    I consider, that you are not right. I am assured. I can defend the position. Write to me in PM, we will discuss. --- What necessary words... super, a remarkable idea porn online video, 1080p porn online, here porn online granny here here here here here here here here here here

  • tietitok - 2018/10/31

    Matchless topic, it is pleasant to me)))) --- This answer, is matchless online tv porn, milf porn online, here kids porn online here here here here here here here here here here

  • brunanml - 2018/10/31

    You are not right. I can prove it. Write to me in PM, we will discuss. --- You have hit the mark. It seems to me it is good thought. I agree with you. granny porn online, online porn lesbians, here online child porn here here here here here here here here here here

  • ewviosi - 2018/10/31

    Has casually come on a forum and has seen this theme. I can help you council. Together we can find the decision. --- Very good question asian porn online, online schoolgirl porn, here porn movies online here here here here here here here here here here

  • rosurBig - 2018/10/31

    I think, that you are not right. I suggest it to discuss. Write to me in PM. --- I advise to you to come on a site where there is a lot of information on a theme interesting you. Will not regret. online cam porn, online porn channels, here little porn online here here here here here here here here here here

  • rauduPLUS - 2018/10/31

    Yes, really. I join told all above. --- Bravo, what phrase..., a remarkable idea online hd porn, home porn online, here online mature porn here here here here here here here here here here

  • yeezy boost - 2018/10/30

    I enjoy you because of all of your effort on this site. Debby really loves making time for internet research and it is obvious why. A lot of people hear all concerning the dynamic tactic you produce rewarding tricks by means of your web blog and therefore invigorate response from some others about this area so our own daughter is undoubtedly becoming educated a lot of things. Enjoy the rest of the new year. You are always carrying out a pretty cool job.

  • michael kors outlet - 2018/10/29

    Needed to compose you the little remark so as to thank you as before with your magnificent opinions you've documented on this page. This is certainly pretty generous with people like you to provide without restraint what numerous people could possibly have supplied for an e-book to help with making some money on their own, most importantly now that you might have tried it if you ever decided. The concepts additionally served to become great way to recognize that some people have the same passion much like my own to see a great deal more in respect of this issue. I am certain there are millions of more fun situations in the future for folks who take a look at your blog.

  • adidas iniki - 2018/10/28

    I want to point out my affection for your kind-heartedness for visitors who really need guidance on this important matter. Your very own dedication to getting the solution throughout was especially valuable and has always allowed workers like me to get to their targets. Your new informative recommendations denotes so much a person like me and even further to my colleagues. Thanks a ton; from all of us.

  • michael kors - 2018/10/28

    I am also commenting to make you be aware of of the helpful encounter my princess developed checking your web page. She even learned many issues, which include how it is like to possess a wonderful helping mood to let many others without problems thoroughly grasp specific problematic topics. You actually exceeded visitors' expected results. Thanks for churning out these informative, healthy, revealing not to mention unique tips about that topic to Kate.

  • golden goose sale - 2018/10/27

    I and my buddies happened to be analyzing the best ideas from your web site and then I got a horrible feeling I had not expressed respect to you for those tips. Those women are already totally excited to study all of them and have sincerely been taking advantage of them. Many thanks for indeed being well considerate and also for opting for certain brilliant ideas millions of individuals are really desperate to be informed on. Our honest apologies for not saying thanks to you earlier.

  • consisisa - 2018/10/26

    Я думаю, что Вы допускаете ошибку. Могу отстоять свою позицию. Пишите мне в PM, пообщаемся. --- Великолепная фраза интересные биографии, интересное смотреть или источник интересный курган тут здесь тут там сайт на этом сайте сайт на странице источник здесь тут источник продолжение здесь здесь

  • yeezy boost 350 - 2018/10/26

    I wanted to post you that very little remark in order to give many thanks as before for all the spectacular concepts you have shared on this site. It was quite wonderfully generous with people like you to present freely exactly what a number of us would have supplied as an electronic book to generate some money for their own end, mostly considering that you might have done it if you considered necessary. The suggestions as well acted to become good way to fully grasp someone else have the identical dream like mine to learn a whole lot more related to this problem. I think there are many more pleasant times up front for those who scan through your website.

  • tory burch - 2018/10/26

    I precisely needed to thank you very much once more. I am not sure what I might have undertaken without these tips documented by you on that concern. It seemed to be a real terrifying situation for me personally, nevertheless encountering this well-written form you managed it took me to jump with delight. Now i am happy for your assistance and in addition believe you comprehend what a powerful job your are accomplishing instructing some other people through your site. Probably you've never got to know all of us.

  • lebron james shoes - 2018/10/25

    I would like to show thanks to the writer for rescuing me from this matter. After surfing around throughout the the net and meeting ideas which are not helpful, I thought my entire life was well over. Being alive minus the solutions to the issues you've resolved by way of your good article content is a serious case, and the kind which may have in a negative way affected my career if I had not encountered your site. Your talents and kindness in controlling a lot of stuff was invaluable. I am not sure what I would've done if I had not come upon such a thing like this. I'm able to now look ahead to my future. Thank you so much for the impressive and sensible help. I won't be reluctant to endorse the website to anyone who wants and needs direction on this issue.

  • oruatSep - 2018/10/23

    Я извиняюсь, но, по-моему, Вы ошибаетесь. Могу отстоять свою позицию. Пишите мне в PM, обсудим. --- По моему мнению Вы не правы. татуировки интересные, манги интересные или here биография интересная here here here here here here here here here here

  • hosubsSem - 2018/10/23

    посмотрю для разнообразия ... --- Абсурдная ситуация получилась канцелярия интересная, интересное посмотреть или here интересная картинка here here here here here here here here here here

  • mopinscrem - 2018/10/23

    и мне........ --- Я присоединяюсь ко всему выше сказанному. Давайте обсудим этот вопрос. вк интересно, интересная книга и here татуировки интересные here here here here here here here here here here

  • dopquidon - 2018/10/23

    Нечего сказать - промолчите, дабы не засорять тему. --- Фраза удалена интересные сайты, интересные юбки или here интересная here here here here here here here here here here

  • voudcatchkr - 2018/10/23

    The properties leaves --- Quite right! I like this idea, I completely with you agree. stocking online porno, porno watch online, here online porno dp here here here here here here here here here here

  • AltonskymN - 2018/09/26

    bestwebcam.us.com

  • AltonskymN - 2018/09/19

    bestwebcam

  • golden goose outlet - 2018/08/28

    An interesting dialogue is value comment. I feel that it's best to write more on this topic, it won't be a taboo topic however usually people are not sufficient to speak on such topics. To the next. Cheers

  • adidas stan smith uk - 2018/08/27

    WONDERFUL Post.thanks for share..more wait .. ?

  • lacoste outlet - 2018/08/26

    Can I just say what a aid to find someone who truly knows what theyre talking about on the internet. You definitely know easy methods to convey an issue to gentle and make it important. Extra folks have to learn this and perceive this side of the story. I cant consider youre no more popular since you positively have the gift.

  • nike zoom - 2018/08/26

    Youre so cool! I dont suppose Ive learn something like this before. So good to find someone with some unique ideas on this subject. realy thanks for starting this up. this website is something that's wanted on the internet, someone with a little bit originality. useful job for bringing something new to the internet!

  • adidas ultra boost 3.0 - 2018/08/26

    I enjoy you because of your own hard work on this website. Ellie really likes doing investigation and it's easy to see why. I hear all regarding the dynamic medium you offer precious guidance by means of your website and in addition welcome contribution from some others on this content then our daughter is without question studying a lot of things. Take pleasure in the rest of the year. You are always conducting a terrific job.

  • adidas ultra - 2018/08/25

    Once I originally commented I clicked the -Notify me when new comments are added- checkbox and now every time a remark is added I get 4 emails with the same comment. Is there any method you possibly can take away me from that service? Thanks!

  • kyrie 4 - 2018/08/24

    Aw, this was a really nice post. In idea I would like to put in writing like this moreover ?taking time and precise effort to make an excellent article?but what can I say?I procrastinate alot and on no account seem to get something done.

  • hermes belts - 2018/08/24

    An impressive share, I just given this onto a colleague who was doing a little bit analysis on this. And he actually purchased me breakfast because I discovered it for him.. smile. So let me reword that: Thnx for the deal with! But yeah Thnkx for spending the time to discuss this, I feel strongly about it and love studying more on this topic. If possible, as you change into experience, would you mind updating your weblog with more details? It's extremely helpful for me. Huge thumb up for this weblog submit!

  • nike air max - 2018/08/23

    An interesting dialogue is value comment. I feel that you must write more on this matter, it may not be a taboo subject but generally persons are not enough to speak on such topics. To the next. Cheers

  • Adidas NMD x YEEZY 350 White - 2018/08/23

    I wanted to put you the bit of observation so as to thank you so much over again about the spectacular things you've provided on this site. It's extremely open-handed with you to give unhampered all a lot of folks would have offered for an e book to generate some cash on their own, precisely given that you could have done it in the event you decided. The secrets as well served as the great way to comprehend other individuals have the identical fervor really like my personal own to figure out great deal more in regard to this matter. I know there are thousands of more pleasant times ahead for individuals that looked over your blog post.

  • michael kors outlet - 2018/08/22

    There's noticeably a bundle to know about this. I assume you made certain good points in options also.

  • adidas nmd - 2018/08/22

    I precisely wished to say thanks again. I do not know the things that I would've worked on in the absence of the type of secrets discussed by you relating to such subject matter. It was actually an absolute depressing issue for me, nevertheless looking at the very specialised approach you processed the issue forced me to weep over contentment. I am happier for your information and hope that you find out what a great job that you are carrying out instructing others using your blog post. I am sure you haven't met all of us.

  • pandora jewelry - 2018/08/22

    Aw, this was a very nice post. In concept I wish to put in writing like this additionally ?taking time and precise effort to make an excellent article?however what can I say?I procrastinate alot and by no means appear to get one thing done.

  • yeezy boost 350 - 2018/08/21

    This really answered my drawback, thanks!

  • nike air max - 2018/08/21

    There are certainly a whole lot of particulars like that to take into consideration. That may be a great point to deliver up. I offer the thoughts above as normal inspiration but clearly there are questions like the one you convey up where crucial factor will be working in honest good faith. I don?t know if greatest practices have emerged round issues like that, but I am sure that your job is clearly recognized as a good game. Each boys and girls feel the impact of only a second抯 pleasure, for the remainder of their lives.

  • gucci belts - 2018/08/20

    This really answered my downside, thanks!

  • adidas iniki - 2018/08/20

    That is the proper blog for anyone who wants to find out about this topic. You realize a lot its nearly exhausting to argue with you (not that I really would need匟aHa). You undoubtedly put a new spin on a subject thats been written about for years. Great stuff, simply great!

  • yeezy boost 350 - 2018/08/19

    It's best to take part in a contest for among the finest blogs on the web. I'll recommend this web site!

  • yeezy boost 350 - 2018/08/19

    I wish to point out my love for your generosity in support of people that really want help on that situation. Your real dedication to passing the solution around turned out to be unbelievably powerful and has surely made professionals just like me to get to their pursuits. This warm and helpful guide indicates so much a person like me and still more to my fellow workers. With thanks; from each one of us.

  • longchamp bags - 2018/08/19

    This site is known as a stroll-by way of for all the data you wanted about this and didn抰 know who to ask. Glimpse right here, and you抣l definitely discover it.

  • yeezy boost 350 v2 - 2018/08/18

    you have got a great blog right here! would you wish to make some invite posts on my blog?

  • michael kors handbags - 2018/08/17

    WONDERFUL Post.thanks for share..extra wait .. ?

  • adidas nmd - 2018/08/17

    Needed to write you one bit of word to thank you as before for those precious opinions you have provided on this website. It has been really extremely open-handed with people like you giving openly just what a few individuals could possibly have supplied for an electronic book to make some money for themselves, mostly seeing that you could possibly have tried it in the event you desired. These tactics likewise worked to become a easy way to be certain that other individuals have a similar fervor just as my personal own to understand more when considering this problem. I'm sure there are some more pleasant periods ahead for individuals who looked at your website.

  • prada eyeglasses - 2018/08/17

    Once I initially commented I clicked the -Notify me when new comments are added- checkbox and now every time a comment is added I get 4 emails with the same comment. Is there any means you'll be able to take away me from that service? Thanks!

  • jordan 1 off white - 2018/08/16

    This actually answered my downside, thank you!

  • adidas yeezy boost - 2018/08/16

    I in addition to my buddies came viewing the nice guidelines on your website and before long I got a terrible suspicion I never expressed respect to the web blog owner for those tips. These young men came as a consequence excited to read through them and now have actually been enjoying those things. Thank you for being quite accommodating and then for considering variety of notable subjects most people are really eager to be informed on. My personal honest apologies for not expressing appreciation to earlier.

  • moncler jacket - 2018/08/15

    Spot on with this write-up, I really suppose this web site wants much more consideration. I抣l probably be again to read much more, thanks for that info.

  • off white shoes - 2018/08/15

    Once I originally commented I clicked the -Notify me when new feedback are added- checkbox and now each time a remark is added I get 4 emails with the same comment. Is there any manner you possibly can take away me from that service? Thanks!

  • pandora jewelry - 2018/08/14

    Youre so cool! I dont suppose Ive read something like this before. So nice to seek out any person with some authentic ideas on this subject. realy thanks for beginning this up. this website is one thing that is needed on the net, someone with a bit originality. helpful job for bringing something new to the web!

  • adidas stan smith sneakers - 2018/08/13

    That is the precise weblog for anybody who desires to search out out about this topic. You realize so much its virtually arduous to argue with you (not that I actually would want匟aHa). You undoubtedly put a brand new spin on a topic thats been written about for years. Nice stuff, simply great!

  • air max 2017 - 2018/08/13

    Oh my goodness! an amazing article dude. Thanks However I'm experiencing problem with ur rss . Don抰 know why Unable to subscribe to it. Is there anybody getting an identical rss downside? Anyone who knows kindly respond. Thnkx

  • adidas ultra boost - 2018/08/13

    Thank you so much for providing individuals with a very brilliant chance to read in detail from this web site. It is usually so ideal and stuffed with amusement for me personally and my office friends to search the blog at minimum thrice every week to learn the newest tips you have. Not to mention, I'm also actually happy concerning the good techniques you serve. Selected 2 tips in this posting are in fact the most impressive I have had.

Санал болгож байна

© 2018 | Ихнүдэн